A simple, sensitive, rapid and reproducible reversed-phase HPLC method has been developed and validated for estimation of salbutamol sulphate and beclomethasone dipropionate in metered dose inhaler formulation. The assay involved an isocratic elution of these two components on water spherisorb, CN, ( 250 mm × 4.6 mm, 10.0 µm) column using a mobile phase composition of buffer: acetonitrile: (60:40). The flow rate was 1.2 ml/min; column oven temperature 25°C and the analytes monitored at 265 nm. Separation was completed within 10 min. Calibration curves were linear with coefficient correlation (R2) 0.999 over a concentration range of 1.0-14.95 μg/ml for salbutamol sulphate and 0.51-7.58 μg/ml for beclomethasone dipropionate respectively. All the validation parameters were within the acceptance range according to ICH norms. The Method has been successfully applied for analysis of drugs in pharmaceutical formulation. Results of analysis were validated statistically and by recovery studies.
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